Up to 86% of metastasised breast tumour (stage 4) retained elevated phosphorylation on both PDK-1(S241) and AKT(308) whereas 100% of stage 3 and 76% of stage 4 revealed moderate to high level of phosphorylation on p70S6K(T389) (Table 3). This evidence concerns the gene RPS6KB1 and breast neoplasm.