When the cytotoxic activity of the CTL line was tested against a large panel of allogeneic cell lines of melanoma, glioma, breast or colorectal carcinoma, autologous or allogeneic Epstein-Barr virus (EBV)-transformed B cells, or autologous fibroblasts, the autologous and a few allogeneic, HLA non-matched melanoma cells were lysed and induced interferon (IFN)-γ and granulocyte monocyte-colony stimulating factor (GM-CSF) secretion by the CTL. This evidence concerns the gene IFNG and central nervous system cancer.