The two BAC clones that detected the highest amplification levels, RP11-110D4 and RP11-195G14, were located approximately 400 kb distally of APOA2. Partial mapping of the amplicon in two other tumours confirmed that the most amplified part was covered by these two BACs (Figure 1C), which detected moderate to high-level amplification in eight of the 10 sarcomas analysed (Figure 1B). This evidence concerns the gene APOA2 and neoplasm.