TSPY1 and hepatocellular carcinoma: Of the eight HCC cell lines tested (HLE, Hep3B, hep-hcc-1, hep-hcc-2, hep-hcc-3, hep-hcc-4, hep-hcc-5, hep-hcc-6), TSPY mRNA was detected in hep-hcc-1 and hep-hcc-4 cell lines (Figure 1C).