In contrast, suppression of HIF1α in neural crest–derived A2058 melanoma cell lines stably expressing HIF1α short hairpin RNA (shRNA) prevented the reduction of SDHB after exposure to cobalt chloride (Figure 4C), in contrast to cells expressing a control shRNA sequence, supporting a central role of HIF1α in regulating SDHB levels. This evidence concerns the gene HIF1A and melanoma.