In addition, the expression of genes whose proteins have previously been shown to be produced by both ovarian carcinoma and stromal cells [28-30], including TIMP-2, vimentin and basic fibroblast growth factor separated the primary tumors from the effusions, suggesting that the cancer-stroma crosstalk is associated with different biological pathway activation than that observed in effusions (Figure 4C, panel c and supplementary Table S2.xls). The gene discussed is TIMP2; the disease is ovarian carcinoma.