SPAM1 and metastatic malignant neoplasm: Northern blot analysis and the more sensitive RT-PCR technique detected the PH-20 transcript in testis, normal breast tissue, metastatic cancer cell lines, fetal and placental cDNA libraries, and murine kidneys, as well as in trace amounts in the prostate, but those studies never investigated the RNA from articular cartilage, synovium, or skin [17-21].