Furthermore, this missense mutation did not segregate with the phenotype in familial melanoma (Hussussian et al, 1994; Harland et al, 1997), and is situated outside the critical four ankyrin repeat domains of p16, and thus does not appear to have any effect in vitro on binding to CDK4 (Ranade et al, 1995; Lilischkis et al, 1996; Harland et al, 1997). This evidence concerns the gene CDKN2A and familial melanoma.