A previous study of CCL2 expression in seven ovarian cancer cell lines by RT–PCR and ELISA, found the highest levels in PEO14 and HL60, with lower levels in OVCAR3, PEO1 and PEO4 and barely detectable expression in SKOV3 and PEA2 cells (Negus et al, 1995). This evidence concerns the gene CCL2 and ovarian cancer.