We next incorporated three specific mutations into the histidine motif region of TRZ1: (i) M535T, which corresponds to the missense substitution A541T in ELAC2 that is associated with human prostate cancer; (ii) H542ter, which corresponds to the frameshift 1641insG associated with human prostate cancer; and (iii) G548R, which corresponds to a ts mutant, G256R, that was isolated in the homologous yeast gene PSO2 [16] (Figure 2) (trz1 M535T, trz1 H542Ter, trz1 G548R; Table 4). Here, DCLRE1A is linked to Familial prostate cancer.