MSH2 and endometrial cancer: While ER α-GAL4 fusion protein (GAL-ER α AF-2) showed a ligand-dependent transactivation function in human HEC59 endometrial cancer cells, a transient coexpression of hMSH2 led to an approximately 2.5-fold increase in the luciferase activity compared to that of GAL-ER α AF-2 alone (Figure 3).