IGKV2D-24 and small cell lung carcinoma: To allow for a direct comparison of expression levels of α7 mRNA between a representative SCLC cell line (NCI-H82), and human SAE cells from a non-smoker (information provided by Clonetics), a riboprobe was transcribed from the PCR fragment amplified by the gene specific α7 primer used for RT-PCR and was used in a nuclease protection assay.