Our finding that hypermethylation of the GTL2 promoter and upstream DMR is associated with silencing of GTL2 and upregulation of DLK1 without LOI would be consistent with a model, whereby GTL2-associated transcripts negatively regulate DLK1 transcription and further analysis of neuroblastoma cell lines with GTL2 hypermethylation and silencing may provide important insights into the mechanisms of imprinting control in the 14q32 imprinted domain. This evidence concerns the gene DLK1 and neuroblastoma.