All cases with GTL2 promoter DMR methylation also demonstrated hypermethylation at the upstream IG-DMR region, but epigenetic alterations were not detected in the three CpG-rich regions close to DLK1. Analysis of DLK1 and GTL2 expression in four neuroblastoma cell lines with GTL2 DMR hypermethylation demonstrated repression of GTL2 transcription and re-expression of GTL2 after treatment with 5-AzaC. This evidence concerns the gene DLK1 and neuroblastoma.