We identified hypermethylation of the GTL2 DMRs in tumours and cell lines, so there are apparent similarities between epigenetics at the H19 and GTL2 loci in human neoplasia, although the frequency of GTL2 hypermethylation in Wilms' tumour is much less than that at H19. However, whereas de novo hypermethylation of the H19 promoter and CTCF box DMRs is associated with H19 silencing and LOI of IGF2, LOI of DLK1 was not associated with GTL2 promoter DMR and IG-DMR hypermethylation. This evidence concerns the gene MEG3 and Wilms tumor.