CENPA and calcinosis: During prometaphase, Sgo1 was concentrated, usually as a single focus, on the inner side of the twin (sister kinetochore) structures stained by a CREST (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, telangiectasias) antiserum (see Figures 1C, part b, and 2A) thought to detect the kinetochore proteins CENP-A/B or C, and Sgo1 overlapped with and covered a slightly larger area than Aurora B during prometaphase (Figure 2B).