ASZ1 and infection: Bertolotti-Ciarlet et al. found that a nucleotide point mutation in a NoV rVP1 construct (ORF2-AUG → ACG-ORF3+3' UTR construct, represented in bold) had decreased levels of rVP1 mRNA at 36 hours post-infection, by approximately 50%, when compared to a construct without the mutation (ORF2+ORF3+3' UTR construct).