TFPI2 and neoplasm: The methylation status of TFPI-2 promoter within the tumour was studied by RE-PCR using two different methyl-sensitive restriction enzymes, HgaI and EagI. When the TFPI-2 gene promoter was not methylated, such as in normal trophoblast cells, a faint band was obtained after PCR with HgaI- or EagI-digested DNA (Figure 5A).