Then we examined expression of sVEGFR-1 and VEGFR-1 by Western blot analysis in 15 randomly selected primary breast cancer tissues prepared with different lysis buffers: 11 tumours had sVEGFR-1 expressions and its expressions were more dominant than VEGFR-1 expressions, and the concentration of sVEGFR-1 measured by ELISA was well correlated (data not shown). The gene discussed is FLT1; the disease is breast cancer.