In our result, the expression of pro-caspase protein was in inverse proportion to the concentrations of AZ-1 (Fig. 8, lane 2), and compared the enzyme activity assay of caspase-3 that was in direct proportion to AZ-1 concentration treatment to BC-M1 cells for 24 hours, as observed in Fig. 9. This evidence concerns the gene CASP3 and breast cancer.