To study the role of Id-1 on ovarian cancer cell growth and its association with EGFR pathway, in this study, we first transfected an Id-1 expression vector into three ovarian cancer cell lines and then examined the effect of ectopic Id-1 expression on ovarian cancer cell proliferation using bromodeoxyuridine (BrdU) staining and flow cytometric analysis. This evidence concerns the gene EGFR and ovarian carcinoma.