TNF and neoplasm: This is supported by the findings that both the Ova-4- and the Ova-5-ACE induced CTL, although exerting weak cytotoxic activity against the HLA-A2.1+ HER-2/neu− autologous primary tumours and against the HLA-A2.1+ HER-2/neu+ tumour cell lines, still were capable of (i) efficiently lysing T2 targets pulsed with the same ACE (i.e. those prepared from Ova-4 and Ova-5 primary tumours), and (ii) producing substantial amounts of IFN-γ and TNF-α after the restimulation phase.