The data from Figure 2 clearly show that IFN-γ and TNF-α levels produced by the MEAMNC bulk population sensitised with ACE from the HER-2/neu− Ova-4 and Ova-5 primary tumours (in ng ml−1: 20.4 and 7.2 for IFN-γ; 2.9 and 5.7 for TNF-α, respectively) were almost within the range of concentrations quantitated in the supernatants of MEAMNC cultures stimulated by ACE from the HER-2/neu+ Ova-1, Ova-2 and Ova-3 primary tumours (range: 7.6–17.3 for IFN-γ and 1.3–10.5 for TNF-α). This evidence concerns the gene ACE and neoplasm.