SERPINA1 and hepatocellular carcinoma: To examine the effect of the degranulated PMN-conditioned media alone or supplemented with exogenously added native AAT (0.5 mg/ml) or its C-36 peptide (0.06 mg/ml) on lung cancer HCC cell proliferation, we measured DNA biosynthesis using a [3H]-thymidine incorporation assay (Fig. 1).