We also showed that E2-2 is the preferential binding partner of the pro-neuronal bHLH protein HASH-1 in SH-SY5Y neuroblastoma cells [42], and the EMSA results presented in this paper, showing that E2-2 is the main E-protein binding a μE5-E-box, corroborate this observation. This evidence concerns the gene ASCL1 and neuroblastoma.