INS and Hypoglycemia: If a double antibody technique is employed (using RIA), the insulin antibodies will increase immunoreactive insulin by competing with anti-insulin antibodies.2 On the other hand, an immunoradiometric assay (IRMA) may have lower false-positive rates and, therefore, be more reliable.2 Hypoglycemia is supposed to be caused by the binding and release of the insulin from the antibodies, which occur out of synchrony with the prevailing glucose concentration.2