To evaluate whether NHE1 is upstream of actin reorganization during serum deprivation in cancer cells, we followed the serum deprivation-dependent remodelling of the F-actin cytoskeleton (Fig. 2a) and quantity of F-actin (Fig. 2b,2c) in the presence or absence of the specific NHE1 inhibitor DMA. The gene discussed is SLC9A1; the disease is cancer.