Since Sp8 was found in human osteosarcoma cell lines and is important in skeletal development [9,10] we examined the regulation of Sp8 by BMP-2 in the murine pluripotent embryonic cell line, C3H10T1/2 that can be induced to form a chondrogenic/osteoblastic phenotype by treatment with BMPs [19-21]. This evidence concerns the gene BMP2 and osteosarcoma.