The proteins were blotted onto a Hybond P PVDF membrane (Amersham Pharmacia Biotech) and incubated with sera from 24 cancer patients that were positive for anti-PDXP in RIA or immunised rabbit-serum diluted 1 : 100 in blocking buffer (PBS with 4% fat-free dry milk) and then with alkaline phosphatase-conjugated rabbit anti-human IgG (Sigma-Aldrich) and goat anti-rabbit IgG (Southern Biotechnology, Birmingham, AL, USA), diluted 1 : 3000 in blocking buffer. The gene discussed is PDXP; the disease is cancer.