For the MYCN amplicon, the largest contiguous region was 1.7 Mb and bounded by NAG and an EST (clone: 757451) in three tumor samples, whilst the smallest region was 27 Kb including an EST (clone: 241343), NCYM, and MYCN. We identified 9 previously reported co-amplified genes (HPCAL1, ODC1, NSE1, NAG, DDX1, NCYM, POMC, DNMT3A, ALK, MEIS1, TEM8) [16,20-27], and detected the novel amplification of several known genes (NCOA1, ADCY3, PPP1CB, CGI-127, LBH, CAPN13, GalNac-T10, EHD3, XDH, SRD5A2, CGI-27, AMP18) and ESTs. The gene discussed is HPCAL1; the disease is neoplasm.