EMD and Emery-Dreifuss muscular dystrophy: Wild-type emerin and EDMD disease-causing mutants S54F and P183H, and alanine substitution mutant m196 (Ser196Ser197 to Ala196Ala197; Holaska et al. 2003), bound efficiently to an F-actin column, whereas 12 other tested mutants, including EDMD disease-causing mutant Q133H, showed no significant binding to F-actin (Figure 1E).