For instance, when randomly selected gp100 / endogenous reference gene Log2 ratios from 10 melanoma metastases were compared with those from 10 kidney specimens only NEDD8, RAb3, FZD6, AFAP, COL8, SNRPD and pooled endogenous reference gene-based normalizations yielded significant differences between the two groups while non significant values were obtained for normalizations based on HIRIP5, β-actin and GPLD1. This evidence concerns the gene AFAP1 and melanoma.