However, while the IRES from EMCV or HTLV-1 could interact with other IRESes present within the retroviral construct (the IRES from gag or the IRES from EMCV), we found that the IRES from FGF-2 induced the expression of exogenous cDNA in human melanoma cells without any positive or negative regulation from the other IRESes. The gene discussed is FGF2; the disease is melanoma.