In this study, we collected the survival data of 351 cases of NSCLCs and correlated the methylation status of five genes (p16INK4a, RASSF1A, APC, RARβ and CDH13) with clinicopathological factors to investigate the effect of methylation of five genes on the survival of patients undergoing curative intent surgical resections for lung cancer. This evidence concerns the gene RASSF1 and lung cancer.