By MSP, CpG island of the Dkk-3 promoter was revealed to be highly methylated in all ALL cell lines (Figure 1A) and in 60 of 183 (33%) samples from patients with ALL (Figure 1C), which was clearly in contrast to no Dkk-3 methylation in normal cells. The gene discussed is DKK3; the disease is acute lymphoblastic leukemia.