DKK3 and acute lymphoblastic leukemia: Exposure to different concentration of the demethylating agent 5-Aza-2′-deoxycytidine restored, in both cell lysate and culture supernatant, the expression of Dkk-3 protein in the MY ALL cell line indicating that hypermethylation is a major mechanism by which Dkk-3 expression is silenced in ALL cells (Figure 1D).