Although there have been many reports on c-myc amplification in human breast cancer (Liao and Dickson, 2000), there are only two published studies involving application of the FISH technique to unfixed, frozen sections (Persons et al, 1997; Visscher et al, 1997), and one prior study using FISH on an archival human tissue microarray (Schraml et al, 1999). This evidence concerns the gene MYC and breast cancer.