Figure 2 shows results of c-Myc in situ hybridisation and immunohistochemistry studies on samples considered to demonstrate low, moderate and high levels of c-Myc expression. Analysis of c-Myc protein localisation results in the nucleus or cytoplasmic compartments of normal and invasive cells within the tumours revealed that nuclear staining was positive in 41% of normal cells, compared to 22% of invasive cells (statistical significance at P=0.01 by McNemar's two-sided χ2 test). This evidence concerns the gene MYC and neoplasm.