To evaluate whether CD40-retargeted Ad infection was accompanied by maturation of CML-DC, as we previously showed for MoDC, we infected CML-DC with untargeted or CD40-targeted Ad, and compared both their phenotype and function with uninfected CML-DC, CML-DC infected by epidermal growth factor receptor (EGFR)-retargeted Ad (using a control immunoconjugate), or CML-DC infected by liposome-complexed Ad (lipofectamin-Ad), which facilitated high-efficiency Ad infection comparable to CD40 retargeted Ad. The gene discussed is CD40; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.