The AFP promoter has been used to activate a suicide gene in HCC cell lines (Kanai et al, 1996; Mawatari et al, 1998), to produce a hybrid promoter in combination with other regulatory regions (Ido et al, 2001) and to apply to oncolytic adenovirus (Hallenbeck et al, 1999). This evidence concerns the gene AFP and hepatocellular carcinoma.