AFP and hepatocellular carcinoma: We thereby examined the transcriptional activity of four kinds of reporter constructs (MK2.3-luc, MK0.6-luc, AFP0.2-luc and AFPEn0.2-luc) in AFP-producing (HuH-7 and PLC/PRF/5) and -nonproducing (HLE and HLF) HCC cell lines (Niwa et al, 1996; Ishikawa et al, 1999), and in non-HCC cell lines (MCF-7 and AsPC-1), which were positive for MK expression (Miyauchi et al, 2001).