We used this technique to measure mRNA levels for wild-type PAX3, PAX7, PAX3-FKHR and PAX7-FKHR in RMS, to determine whether higher levels of wild-type PAX3 and PAX7 are observed in tumours that do not express PAX3-FKHR and PAX7-FKHR chimaeric transcripts. This evidence concerns the gene PAX7 and neoplasm.