GAPDH and tarsal-carpal coalition syndrome: Figure 3E demonstrates that hAES was expressed in all TCC lines, normal urothelial cells and HepG2, except for BFTC905 showing only weak expression. In normal urothelial cells, a second faint band could be observed, which may correspond to a splice variant. As for hTCF1, both inducible TCC lines displayed slightly increased hAES expression relative to GAPDH.