To show the effect of CPT-11 conversion into SN-38 by CE2, sCE2 and C28-sCE2 on the viability of colon cancer cells, the EpCAM-expressing colon carcinoma cell line SW1398 was incubated overnight with the concentrated supernatant of COS-7 cells transfected with pCE2, psCE2 or pC28-sCE2. This evidence concerns the gene EPCAM and colon carcinoma.