Two cDNAs (LIM kinase and BUB3), which are differentially-expressed in the present malignant library, have also been found to be differentially expressed in a subtracted library representing cDNAs expressed in a pathologically homogeneous breast carcinoma specimen subtracted with cDNA from 50 000 ductal-carcinoma-in-situ cells microdissected from the surrounding normal tissue (Liu, 2001). The gene discussed is BUB3; the disease is breast carcinoma.