To test whether a reduction in VEGF expression by tumour cells could affect endothelial cell migration, HMVEC-L or MHE cells were stained with 10 μg ml−1 Di-I for 24 h and added into Fluoroblok inserts placed into 24 well dishes containing Caki-1 tumour cells treated with either control or V515. This evidence concerns the gene VEGFA and neoplasm.