Fajardo et al (1988) also reported that hyperthermia inhibits angiogenesis by interference with cell replication and/or inhibition of the migration of vascular endothelial cells. However, the effect of hyperthermia on the production of VEGF is not specified. In the present study, we demonstrated for the first time that heat shock suppresses the gene expression and the production of VEGF165 in HT-1080 cells. Moreover, the heat shock-mediated suppressions of both VEGF165 production and its gene expression were similarly observed in human squamous carcinoma A431 cells (data not shown). The gene discussed is VEGFA; the disease is squamous cell carcinoma.