PLAU and prostate carcinoma: Until now there has been no human in vitro model to demonstrate the role of proteolytic enzymes on metastasis formation, and there have been few studies to determine whether blocking these enzymes causes significant effect (although, previous animal models have shown that rats inoculated with Dunning R 3227, Mat LyLu rat prostate carcinoma cell line transfected to over-express uPA developed hind limb paralysis much faster than transfectants which under-expressed uPA (Achbarou et al, 1994)).