S100A4 and colon carcinoma: When the colon carcinomas were subdivided according to the Duke's classification, the S100A4 mRNA levels for class A (non invasive), class B (invasive with no nodal involvement), class C (metastasis to regional lymph nodes) were not significantly different (A vs B, P=0.933; A vs C, P=0.888; B vs C, P=0.11; 2-tailed Mann–Whitney U-Test.