CCND1 and breast neoplasm: To determine the prognostic value of CCND1 amplification and/or overexpression, we used this real-time PCR method to measure CCND1 gene expression at the mRNA level in a large series of unilateral invasive primary breast tumours (n=134) with known CCND1 gene status (Bièche et al, 1998) and available long-term outcome data.