All 134 tumours studied for CCND1 expression at the RNA level had previously been tested for CCND1 amplification by Southern blot analysis (unpublished data), and 94 had also been tested (when DNA was still available) with a real-time quantitative PCR assay based on TaqMan technology (Bièche et al, 1998). Here, CCND1 is linked to neoplasm.