The CCND1 mRNA and DNA status of these three tumours was confirmed by conducting a second RNA and DNA extraction, by additional real-time quantitative PCR and RT–PCR analyses (use of new primer pairs for the CCND1 and TBP genes, and an additional endogeneous RNA control; the RPLP0 gene (also known as 36B4) encoding human acidic ribosomal phosphoprotein P0) and by Northern and Southern analysis. This evidence concerns the gene TBP and neoplasm.