The present study demonstrated the presence of Snail mRNA in HuL-1, Changliver, HLE and HLF cells, but not in Hep-G2 cells detected by RT–PCR, these patterns were further proved by ISH in tumours, where Snail mRNA signals expressed in each tumour sections induced by HuL-1, Changliver, and HLF, respectively. The gene discussed is SNAI1; the disease is neoplasm.