Quantitative gene profiling of long noncoding RNAs with targeted RNA sequencing.
(PMID:25751143)
PMID:25751143
Michael Clark
1] Garvan Institute of Medical Research, Sydney, Australia. [2] MRC Functional Genomics Unit, Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK.
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Tim R Mercer
1] Garvan Institute of Medical Research, Sydney, Australia. [2] St Vincents Clinical School, Faculty of Medicine, University of New South Wales, Sydney, Australia.
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Giovanni Bussotti
Tommaso Leonardi
Katelin R Haynes
The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, Australia.
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Joanna Crawford
Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia.
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Marion E Brunck
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Australia.
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Kim-Anh Lê Cao
1] The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, Australia. [2] Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia.
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Gethin P Thomas
The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, Australia.
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Wendy Y Chen
1] Garvan Institute of Medical Research, Sydney, Australia. [2] St Vincents Clinical School, Faculty of Medicine, University of New South Wales, Sydney, Australia.
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Ryan J Taft
1] Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia. [2] Illumina, Inc., San Diego, California, USA. [3] School of Medicine and Health Services, Departments of Integrated Systems Biology and Pediatrics, George Washington University, Washington DC, USA.
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Lars Nielsen
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Australia.
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Anton Enright
EMBL - European Bioinformatics Institute
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John Mattick
Garvan Institute of Medical Research
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Marcel Dinger
1] Garvan Institute of Medical Research, Sydney, Australia. [2] St Vincents Clinical School, Faculty of Medicine, University of New South Wales, Sydney, Australia.
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Affiliations
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1. 1] Garvan Institute of Medical Research, Sydney, Australia. [2] MRC Functional Genomics Unit, Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK.
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2. 1] Garvan Institute of Medical Research, Sydney, Australia. [2] St Vincents Clinical School, Faculty of Medicine, University of New South Wales, Sydney, Australia.
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3. European Molecular Biology Laboratory-European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Cambridge, UK.
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4. The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, Australia.
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5. Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia.
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6. Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Australia.
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7. 1] The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, Australia. [2] Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia.
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8. 1] Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia. [2] Illumina, Inc., San Diego, California, USA. [3] School of Medicine and Health Services, Departments of Integrated Systems Biology and Pediatrics, George Washington University, Washington DC, USA.
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Nature Methods
[09 Mar 2015, 12(4):339-342]
2015/05
Abstract
We compared quantitative RT-PCR (qRT-PCR), RNA-seq and capture sequencing (CaptureSeq) in terms of their ability to assemble and quantify long noncoding RNAs and novel coding exons across 20 human tissues. CaptureSeq was superior for the detection and quantification of genes with low expression, showed little technical variation and accurately measured differential expression. This approach expands and refines previous annotations and simultaneously generates an expression atlas.
